Total Starch Assay Kit (AA/AMG)

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products.  This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

Advantages:

• Very cost effective
• All reagents stable for > 12 years after preparation
• Simple format
• Mega-Calc™ software tool for hassle-free raw data processing
• Standard included

Principle:

• (α-amylase, 100°C ± DMSO)
• (1) Starch granules + H2O → maltodextrins
• (amyloglucosidase)
• (2) Maltodextrins + H2O → D-glucose
• (glucose oxidase)
• (3) D-Glucose + H2O + O2 → D-gluconate + H2O2
• (peroxidase)
• (4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
• quinoneimine + 4H2O
• Kit size: 50/100 assays
• Method: Spectrophotometric at 510 nm
• Total assay time: ~ 90 min
• Detection limit: 1-100% of sample weight